Identification of markers of infection in the breath using a pig pneumonia model
Presented During:
Saturday, May 6, 2023: 8:00AM - Tuesday, May 9, 2023: 11:45AM
Los Angeles Convention Center
Posted Room Name:
Outside of Room 408
Abstract No:
PS057
Submission Type:
Abstract Submission
Authors:
Victor van Berkel (1), Gianna Katsaros (1), Susan Ansley Smith (1), Sienna Shacklette (2), Zhenzhen Xie (2), Xiao-An Fu (2), George Pantalos (1)
Institutions:
(1) University of Louisville School of Medicine, Louisville, KY, (2) University of Louisville Speed School of Engineering, Louisville, KY
Submitting Author:
Victor van Berkel
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University of Louisville School of Medicine
University of Louisville School of Medicine
Co-Author(s):
Gianna Katsaros
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University of Louisville School of Medicine
University of Louisville School of Medicine
Susan Ansley Smith
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University of Louisville School of Medicine
University of Louisville School of Medicine
Sienna Shacklette
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University of Louisville Speed School of Engineering
University of Louisville Speed School of Engineering
Zhenzhen Xie
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University of Louisville Speed School of Engineering
University of Louisville Speed School of Engineering
Xiao-An Fu
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University of Louisville Speed School of Engineering
University of Louisville Speed School of Engineering
George Pantalos
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University of Louisville School of Medicine
University of Louisville School of Medicine
Presenting Author:
Gianna Katsaros
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University of Louisville School of Medicine
University of Louisville School of Medicine
Abstract:
Objective: Pneumonia, both in the community and hospital setting, represents a significant cause of morbidity and mortality in the cardiothoracic patient population. Diagnosis of pneumonia can be masked by other disease processes, and is often diagnosed after the patient is already suffering from the disease. A non-invasive, sensitive test for pneumonia, which would allow for treatment before clinical deterioration, would decrease hospitalizations and length of stay for patients. We have developed a porcine model of pneumonia, and evaluated the exhaled breath of infected pigs for biomarkers of infection.
Methods: 60kg adult pigs were intubated and a bronchoscope was used to instill either a solution containing 12 x 10^8 cfu of methicillin sensitive staph aureus (MSSA), or a control solution without bacteria (SHAM), into the distal airways. The pigs were then re-intubated on POD#3, #6, and #9, with bronchoscopic bronchial lavages taken at each time point. At each time point, a 500cc breath was captured from each pig. The breath was evacuated over a silicon microchip, with the contents of the breath captured via oximation reaction, and the results of this capture were analyzed by mass spectroscopy.
Results: The pigs infected with MSSA demonstrated clinical signs of infection (initial fever and persistent cough), demonstrated consolidation on CXR, and showed increasing counts of MSSA in the bronchial lavages over the span of the experiment. Analysis of the exhaled breath demonstrated one carbonyl compound (Unsaturated 2-pentanal) that increased 10-fold over the span of the experiment, from an average of 0.0294 nmols/L before infection to an average of 0.3836 nmol/L on POD#9. The sham infected pigs showed no significant change in this compound over the same time frame.
Conclusions: We were able to successfully develop a clinical pneumonia in adult 60kg pigs. Unsaturated 2-pentanal functions as a biomarker for MSSA infection in pigs, demonstrating the potential utility of this technology for early diagnosis of pneumonia.
Methods: 60kg adult pigs were intubated and a bronchoscope was used to instill either a solution containing 12 x 10^8 cfu of methicillin sensitive staph aureus (MSSA), or a control solution without bacteria (SHAM), into the distal airways. The pigs were then re-intubated on POD#3, #6, and #9, with bronchoscopic bronchial lavages taken at each time point. At each time point, a 500cc breath was captured from each pig. The breath was evacuated over a silicon microchip, with the contents of the breath captured via oximation reaction, and the results of this capture were analyzed by mass spectroscopy.
Results: The pigs infected with MSSA demonstrated clinical signs of infection (initial fever and persistent cough), demonstrated consolidation on CXR, and showed increasing counts of MSSA in the bronchial lavages over the span of the experiment. Analysis of the exhaled breath demonstrated one carbonyl compound (Unsaturated 2-pentanal) that increased 10-fold over the span of the experiment, from an average of 0.0294 nmols/L before infection to an average of 0.3836 nmol/L on POD#9. The sham infected pigs showed no significant change in this compound over the same time frame.
Conclusions: We were able to successfully develop a clinical pneumonia in adult 60kg pigs. Unsaturated 2-pentanal functions as a biomarker for MSSA infection in pigs, demonstrating the potential utility of this technology for early diagnosis of pneumonia.
THORACIC:
Basic and Translational Research
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