Structural and Enzymatic Comparison of Faecalibacterium prausnitzii GH31 α-glycosidases

Presented During:

07/30/2022: 5:30 PM - 7:30 PM
Portland Marriott Downtown Waterfront  


2022: 72nd ACA Annual Meeting

Session Type:


Presenting Author :

Anna Jewczynko  
University of Waterloo

Additional Author:

David Rose  
Dept of Biology, Univ of Waterloo

Abstract Body:

The gut microbiome is home to thousands of species of bacteria, that are essential for human digestion, immunity, and physiology. Faecalibacterium prausnitzii makes up about 5% of a healthy human gut microbiome and a lower abundance of this bacterium has been found in patients with IBD and Crohn's disease. Among an extensive repertoire of carbohydrate active enzymes, F. prausnitzii has 2 GH31 α-glycosidases, which are from the same family as Sucrase-Isomaltase and Maltase-Glucoamylase, human digestive enzymes with overlapping and distinguishing substrate specificities. This project aims to characterize the substrate specificity and preference of F. prausnitzii GH31 α-glycosidases to better understand the structural features of GH31 enzymes and the biological capabilities of these bacteria. AlphaFoldV2.1.0 was used to create computational models of F. prausnitzii α-glycosidases, and the substrate specificity and kinetics parameters are reported. Structurally, these α-glycosidases have the same identified conserved N-terminal and (β/α)8 barrel domains, but FpAG1 has an additional conserved domain of unknown function at the C-terminus which is not found in the FpAG2 structure. Both FpAG1 and FpAG2 have α-glucosidase and oligo-1,6-glucosidase activity. The comparative kinetic studies show that FpAG1 has a greater preference for α-1,6 glycosidic linkages, and FpAG2 has a greater preference for α-1,4 glycosidic linkages. Gaining insight on the GH31 α-glycosidases as a component of F. prausnitzii metabolism can further our understanding of this community in the human gut microbiome.

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