H11: Reduction of Clostridioides Difficile Spores Using Titanium Nanocomposite

Phoenix Convention Center 

CM Credit Hours: 1 

Clostridioides difficile (C. diff.) is a bacterium that can cause severe gastrointestinal infections, particularly in hospitalized patients who have recently taken antibiotics. C. diff. Infection (CDI) can lead to symptoms such as diarrhea, often accompanied by abdominal pain, cramping, and fever. In some severe cases, CDI can lead to complications such as sepsis, kidney failure, and even death. We are using nanoparticles for C. diff treatments. We are trying to reduce C. diff from environmental contaminations. The bacterium can form spores that are highly resistant to environmental stressors and can survive for long periods on surfaces. Therefore, proper cleaning and disinfection protocols can help reduce the risk of transmission and infection. However, some of the issues with traditional disinfection are that it requires a high volume to treat the bacteria and can be hazardous to human skin. Also, a high frequency of application needs to be adequately effective, which can cause demanding labor and a dangerous worker environment. Nanoparticles are tiny particles with a diameter of fewer than 100 nanometers. They have unique properties that can be utilized for various applications. For example, titanium dioxide (TiO2) nanoparticles have been shown to have antimicrobial properties and can damage the cell walls of bacteria, including C. diff. In this study, we will manufacture TiO2 nanocomposites and investigate their ability to reduce C. diff spores on surfaces. We will use various surface amounts of titanium dioxide nanocomposite to test the reduction rates of C. diff on the surface. We will culture C. diff spores in the biosafety lab and dispense the spore mixture into various nanocomposites with different TiO2 concentrations. After 24 hours, serial dilution will be repeated on media, and colonies will be counted following incubation. Finally, we will use ANOVA to determine the difference between the control disc and TiO2 nanocomposites.

Upon Completion, the participant will be able to address the effect of the various nanocomposites with different Titanium (TiO2) Concentrations on Clostridioides difficile (C. diff) spore. 

Introductory

Academia/Education

Engineering

Healthcare/Pharma

Laboratories

Academic Track

Aerosols & Airborne Particulates

Biosafety/Microbiology

Nanotechnology